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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
12/08/2022 |
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Data da última atualização: |
12/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
IBELLI, A. M. G.; PEIXOTO, J. de O.; ZANELLA, R.; GOUVEIA, J. J. de S.; CANTAO, M. E.; COUTINHO, L. L.; MARCHESI, J. A. P.; DAL PIZZOL, M. S.; MARCELINO, D. E. P.; LEDUR, M. C. |
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Afiliação: |
ADRIANA MERCIA GUARATINI IBELLI, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; RICARDO ZANELLA, UPF; JOÃO JOSÉ DE SIMONI GOUVEIA, UNIVASF; MAURICIO EGIDIO CANTAO, CNPSA; LUIZ LEHMANN COUTINHO, Esalq; JORGE AUGUSTO PETROLI MARCHESI, USP; MARIANE SPUDEIT DAL PIZZOL, UDESC/Chapecó; DÉBORA ESTER PETRY MARCELINO, FACC; MONICA CORREA LEDUR, CNPSA. |
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Título: |
Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Physiology, v. 3, n. 941134, 2022. doi: 10.3389/fphys.2022.941134 |
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DOI: |
https://doi.org/10.3389/fphys.2022.941134 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Femoral head separation (FHS) is characterized by the detachment of growth plate (GP) and articular cartilage, occurring in tibia and femur. However, the molecular mechanisms involved with this condition are not completely understood. Therefore, genes and biological processes (BP) involved with FHS were identified in 21-day-old broilers through RNA sequencing of the femoral GP. 13,487 genes were expressed in the chicken femoral head transcriptome of normal and FHS-affected broilers. From those, 34 were differentially expressed (DE; FDR ≤0.05) between groups, where all of them were downregulated in FHS-affected broilers. The main BP were enriched in receptor signaling pathways, ossification, bone mineralization and formation, skeletal morphogenesis, and vascularization. RNA-Seq datasets comparison of normal and FHS-affected broilers with 21, 35 and 42 days of age has shown three shared DE genes (FBN2, C1QTNF8, and XYLT1) in GP among ages. Twelve genes were exclusively DE at 21 days, where 10 have already been characterized (SHISA3, FNDC1, ANGPTL7, LEPR, ENSGALG00000049529, OXTR, ENSGALG00000045154, COL16A1, RASD2, BOC, GDF10, and THSD7B). Twelve SNPs were associated with FHS (p < 0.0001). Out of those, 5 were novel and 7 were existing variants located in 7 genes (RARS, TFPI2, TTI1, MAP4K3, LINK54, and AREL1). We have shown that genes related to chondrogenesis and bone differentiation were downregulated in the GP of FHS-affected young broilers. Therefore, these findings evince that candidate genes pointed out in our study are probably related to the onset of FHS in broilers. MenosAbstract: Femoral head separation (FHS) is characterized by the detachment of growth plate (GP) and articular cartilage, occurring in tibia and femur. However, the molecular mechanisms involved with this condition are not completely understood. Therefore, genes and biological processes (BP) involved with FHS were identified in 21-day-old broilers through RNA sequencing of the femoral GP. 13,487 genes were expressed in the chicken femoral head transcriptome of normal and FHS-affected broilers. From those, 34 were differentially expressed (DE; FDR ≤0.05) between groups, where all of them were downregulated in FHS-affected broilers. The main BP were enriched in receptor signaling pathways, ossification, bone mineralization and formation, skeletal morphogenesis, and vascularization. RNA-Seq datasets comparison of normal and FHS-affected broilers with 21, 35 and 42 days of age has shown three shared DE genes (FBN2, C1QTNF8, and XYLT1) in GP among ages. Twelve genes were exclusively DE at 21 days, where 10 have already been characterized (SHISA3, FNDC1, ANGPTL7, LEPR, ENSGALG00000049529, OXTR, ENSGALG00000045154, COL16A1, RASD2, BOC, GDF10, and THSD7B). Twelve SNPs were associated with FHS (p < 0.0001). Out of those, 5 were novel and 7 were existing variants located in 7 genes (RARS, TFPI2, TTI1, MAP4K3, LINK54, and AREL1). We have shown that genes related to chondrogenesis and bone differentiation were downregulated in the GP of FHS-affected young broilers. Therefore, these... Mostrar Tudo |
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Palavras-Chave: |
FBN2; Femoral head necrosis; FHS; Necrose da cabeça femoral; RNA-Seq. |
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Thesagro: |
Frango; Galinha; Genética Animal. |
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Thesaurus Nal: |
Chickens; Gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145416/1/final9937.pdf
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Marc: |
LEADER 02692naa a2200361 a 4500
001 2145416
005 2022-08-12
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fphys.2022.941134$2DOI
100 1 $aIBELLI, A. M. G.
245 $aDownregulation of growth plate genes involved with the onset of femoral head separation in young broilers.$h[electronic resource]
260 $c2022
520 $aAbstract: Femoral head separation (FHS) is characterized by the detachment of growth plate (GP) and articular cartilage, occurring in tibia and femur. However, the molecular mechanisms involved with this condition are not completely understood. Therefore, genes and biological processes (BP) involved with FHS were identified in 21-day-old broilers through RNA sequencing of the femoral GP. 13,487 genes were expressed in the chicken femoral head transcriptome of normal and FHS-affected broilers. From those, 34 were differentially expressed (DE; FDR ≤0.05) between groups, where all of them were downregulated in FHS-affected broilers. The main BP were enriched in receptor signaling pathways, ossification, bone mineralization and formation, skeletal morphogenesis, and vascularization. RNA-Seq datasets comparison of normal and FHS-affected broilers with 21, 35 and 42 days of age has shown three shared DE genes (FBN2, C1QTNF8, and XYLT1) in GP among ages. Twelve genes were exclusively DE at 21 days, where 10 have already been characterized (SHISA3, FNDC1, ANGPTL7, LEPR, ENSGALG00000049529, OXTR, ENSGALG00000045154, COL16A1, RASD2, BOC, GDF10, and THSD7B). Twelve SNPs were associated with FHS (p < 0.0001). Out of those, 5 were novel and 7 were existing variants located in 7 genes (RARS, TFPI2, TTI1, MAP4K3, LINK54, and AREL1). We have shown that genes related to chondrogenesis and bone differentiation were downregulated in the GP of FHS-affected young broilers. Therefore, these findings evince that candidate genes pointed out in our study are probably related to the onset of FHS in broilers.
650 $aChickens
650 $aGene expression
650 $aFrango
650 $aGalinha
650 $aGenética Animal
653 $aFBN2
653 $aFemoral head necrosis
653 $aFHS
653 $aNecrose da cabeça femoral
653 $aRNA-Seq
700 1 $aPEIXOTO, J. de O.
700 1 $aZANELLA, R.
700 1 $aGOUVEIA, J. J. de S.
700 1 $aCANTAO, M. E.
700 1 $aCOUTINHO, L. L.
700 1 $aMARCHESI, J. A. P.
700 1 $aDAL PIZZOL, M. S.
700 1 $aMARCELINO, D. E. P.
700 1 $aLEDUR, M. C.
773 $tFrontiers in Physiology$gv. 3, n. 941134, 2022. doi: 10.3389/fphys.2022.941134
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
11/05/2001 |
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Data da última atualização: |
30/03/2023 |
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Autoria: |
PRADO, R. de M.; CENTURION, J. F. |
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Afiliação: |
Renato de Mello Prado, Universidade Estadual Paulista - UNESP/Faculdade de Ciências Agrárias e Veterinárias - FCAV/Departamento de Solos e Adubos; José Frederico Centurion, Universidade Estadual Paulista - UNESP/Faculdade de Ciências Agrárias e Veterinárias - FCAV/Departamento de Solos e Adubos. |
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Título: |
Alterações na cor e no grau de floculação de um Latossolo Vermelho-Escuro sob cultivo contínuo de cana-de-açúcar. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 36, n. 1, p. 197-203, jan. 2001 |
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Idioma: |
Português |
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Notas: |
Notas Científicas.
Título em inglês: Alterations of the colour and of the degree of floculation of a Dark-Red Latosol under conditions of continuous cultivation of sugar cane. |
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Conteúdo: |
Objetivou-se avaliar o grau de modificacao de algumas propriedades fisicas e da cor do horizonte superficial de um Latossolo Vermelho-Escuro textura media, submetido ao cultivo contínuo com cana-de-acucar durante 25 anos, em Jaboticabal, SP. Os tratamentos foram constituídos por dois tipos de uso do solo, ou seja, ausência de cultivo (vegetacao nativa) e cultivo intenso com cana-de-acucar e por tres profundidades no perfil, 0-10, 10-20 e 20-30 cm. O cultivo intenso e continuo alterou a cor do solo na camada de 0-10 cm de 2,5YR 2,5/4 na ausencia de cultivo para 2,5YR3/4 e degradou a macroestrutura, reduzindo o grau de floculacao da argila do solo. |
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Thesagro: |
Argila; Cana de Açúcar; Morfologia Vegetal; Saccharum Officinarum; Solo. |
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Thesaurus NAL: |
Clay; Soil morphology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/18926/1/pab99_235.pdf
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Marc: |
LEADER 01541naa a2200229 a 4500
001 1103657
005 2023-03-30
008 2001 bl uuuu u00u1 u #d
100 1 $aPRADO, R. de M.
245 $aAlterações na cor e no grau de floculação de um Latossolo Vermelho-Escuro sob cultivo contínuo de cana-de-açúcar.$h[electronic resource]
260 $c2001
500 $aNotas Científicas. Título em inglês: Alterations of the colour and of the degree of floculation of a Dark-Red Latosol under conditions of continuous cultivation of sugar cane.
520 $aObjetivou-se avaliar o grau de modificacao de algumas propriedades fisicas e da cor do horizonte superficial de um Latossolo Vermelho-Escuro textura media, submetido ao cultivo contínuo com cana-de-acucar durante 25 anos, em Jaboticabal, SP. Os tratamentos foram constituídos por dois tipos de uso do solo, ou seja, ausência de cultivo (vegetacao nativa) e cultivo intenso com cana-de-acucar e por tres profundidades no perfil, 0-10, 10-20 e 20-30 cm. O cultivo intenso e continuo alterou a cor do solo na camada de 0-10 cm de 2,5YR 2,5/4 na ausencia de cultivo para 2,5YR3/4 e degradou a macroestrutura, reduzindo o grau de floculacao da argila do solo.
650 $aClay
650 $aSoil morphology
650 $aArgila
650 $aCana de Açúcar
650 $aMorfologia Vegetal
650 $aSaccharum Officinarum
650 $aSolo
700 1 $aCENTURION, J. F.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 36, n. 1, p. 197-203, jan. 2001
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